KMID : 0364820110470010130
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Korean Journal of Microbiology 2011 Volume.47 No. 1 p.130 ~ p.136
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Characterization of Actinomyces Isolated from Freshwater Sponges in Lake Baikal
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Jung You-Jung
Joung Yo-chan Ahn Tae-Seok
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Abstract
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Five strains of Actinomyces were isolated from freshwater sponges, Baikalospongia and Lubomirskia, in Lake Baikal. By 16S rRNA sequencing, isolates were identified as Streptomyces griseoplanus, S. halstedii, S. violascens, S. flavovirens, and S. microflavus. Isolates had different characteristics of growth temperature, carbon utilization, enzyme activity, and cellular fatty acid composition. Optimum growth conditions of isolates were 30-37¡ÆC, pH 8-9, and 0-1.5% salt oncentrations. Major fatty acid compositions were anteiso-C15:0, iso-C15:0, and iso-C16:0. Strain ATS-BA-19 had DNase and chitinase activities and strain ATS-BA-16 had cellulase and protease activities. Colonies of strain ATS-BA-15 and ATS-BA-19 made inhibition zone of Pseudomonas aeruginosa.
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KEYWORD
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Actinomycetes, fresh water, lake Baikal, sponge
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